Existing resource – a common garden experiment was initiated in October 2022, and fish will be reared initially at a hatchery in Dorion, Ontario, to the eyed egg stage, at which time a sub-sample from families from each of 5 lakes will be transferred to an experimental fish culture facility in Codrington, Ontario. From commencement of the PhD in fall 2023, fish will be available for the collection of phenotypic data under the present project. The three key methodological areas for the student are as follows:
Pedigree reconstruction – the study involves a partial sires within dams design with offspring reared by maternal sibship. The student will collaborate with existing experienced staff to generate a marker panel to recover paternal sibship data such that the pedigree will be fully resolved.
Phenotyping – the student will collect multivariate phenotypic information on body size, morphology, temperature tolerance, and behavioural traits.
Statistical analysis – to assess the extent and nature genetic differentiation and within-population standing variation, and to test hypotheses about the relationship between genetic variation at these two levels, the student will estimate among- and within- population patterns of genetic variation in the phenotyped traits using linear mixed modelling frameworks.