IAP2-22-375

Soil carbon and biodiversity response to Scottish Highland rewilding

Rewilding is increasingly considered as a powerful tool to limit both the ongoing declines in biodiversity and mitigate climate change, through its potential to restore biodiverse habitats and increase ecosystem-scale carbon (C) storage. In Scotland, where ca. seven million sheep and one million deer graze the land predator-free and prevent the natural regeneration of trees, the removal of these large herbivores through fencing has allowed natural forest regeneration in several small-scale experiments (see below). The visual success of these initiatives has been embraced by the rewilding conservation movement and has led to increasingly vocal calls to ‘rewild’ the Scottish Highlands. Rewilding initiatives often infer that there will be wider holistic benefits (e.g., Warner et al. 2021), however, confidence in the true benefits of rewilding, particularly biodiversity recovery and C sequestration, is limited due to a lack of above and belowground data.

While these rewilding initiatives likely increase above ground carbon storage by allowing tree growth, the overall change in ecosystem C storage also depends on the response of the largest C stock in terrestrial ecosystems: the soil C stock. Recent findings from experimental tree planting in the Scottish uplands suggests that while tree planting led to an accumulation of C aboveground, it simultaneously led to decrease in C sequestration belowground (Friggens et al. 2020), thereby cancelling out the aboveground benefits. Preliminary data from a rewilding initiative at Ben Lawers Nature reserve suggest that this decrease in soil C may also occur following the natural regeneration of trees. Furthermore, soils are globally the largest reservoir of biodiversity, hosting more than 25% of the Planet’s total biodiversity (Bardgett and van der Putten (2014). Holistic benefits of rewilding on biodiversity thus largely depend on the response of the soil. There is thus an urgent need to carefully considering the response of the soil to rewilding. Can rewilding the Scottish Highlands increase ecosystem-scale carbon storage and biodiversity, once the soil is considered?

The overall aim of this PhD is to evaluate the carbon and biodiversity benefits of rewilding across a network of rewilding initiatives across Scotland, through a focus on soils and its interactions to the surrounding environment. Specifically, through field sampling and laboratory analyses, this PhD will answer the following questions:
1. How does the amount and stability of soil C respond to rewilding?
2. How does the diversity of soil organisms respond to rewilding?
3. What are the overall ecosystem-scale C benefits, when considering the soil, aboveground, and freshwater compartments?

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Image Captions

Example of a rewilding initiative, here at Ben Lawers National Nature Reserve

Methodology

To answer these questions, the PhD student will select a network of rewilding sites across the Scottish Highlands in collaboration with from Forest Research, where they will use a range of field sampling techniques followed by complementary laboratory experimentation and analyses:
– First, to determine the response of soil carbon to rewilding, soil samples will be collected inside and outside the rewilding areas and analysed to determine the soil C stocks throughout the soil profile, and the form in which the C is stored (dissolved, mineral-associated or particulate organic matter) to inform on C stability, along with standard soil parameters. Further, to determine the response of important soil functions to rewilding, soil cores from paired sites will be incubated under laboratory conditions to measure soil C stability and soil greenhouse gas emission potential.
– Second, to determine the response of soil organisms to rewilding, soil samples will be collected seasonally for one year, inside and outside the rewilding areas, and microorganisms and invertebrates will then be extracted and identified.
– Last, to determine the overall ecosystem-scale C, the response of aboveground plant biomass and freshwater carbon will be measured.

Project Timeline

Year 1

– Review of literature (Questions 1-3)
– Training on field, lab and analytical methods (Questions 1-3)
– Establishment of network of rewilded sites with paired control sites across the Scottish Highlands, and recording of site-specific characteristics as covariates (e.g., soil type, past land-use, topography) (Questions 1-3)
– Field sampling of soils for soil carbon analyses, and estimation of aboveground plant biomass, in collaboration with CASE partner Forest Research (FR) (Questions 1 and 3)
– Soil characterisation at Stirling and UKCEH Lancaster (Question 1)

Year 2

– Set up of soil incubations at Stirling and UKCEH Lancaster (Question 1)
– Seasonal field sampling of soils for soil community determination (microorganisms and invertebrates), combined with seasonal freshwater sampling (Questions 2-3)
– Presentation of key result at national/international conference (Question 1)
– Analysis of data and paper writing (Question 1)
– Further statistical training (Questions 1-3)

Year 3

– Extraction and identification of soil invertebrates with CASE partner FR (Question 2)
– Extraction and determination of soil microorganisms at UKCEH Lancaster (Question 2)
– Analysis of data and paper writing (Question 2)

Year 3.5

– Analysis of data and paper/thesis writing Questions 1-3)
– Presentation of key result at national/international conference (Question 1-3)

Training
& Skills

We are committed to providing the PhD student with a supportive and inclusive working environment, and to supporting the student in gaining the skills and experience in line with their professional aspirations. In this PhD, the student will have multiple opportunities to develop a range of professionally transferable skills, including skills in project design and management, in invertebrate identification, in diverse state-of-the-art field/ experimental/ laboratory methods, in data analyses, as well as in communication, teaching and outreach. These skills will be developed through interactions with the supervisory team, within the Biological and Environment Sciences (University of Stirling) lively and supportive community, through the IAPETUS specific provision, and through external courses. The student will be able to take advantage of experimental approaches and facilities developed by the supervisors in the NERC Locked Up project (Whitaker, Elias) and to spend discrete periods of time in the Plant-Soil Interactions group at UKCEH Lancaster being trained and using a range of plant-soil biogeochemical analyses including trace gas instrumentation (IRGAs and gas chromatography), analysis of microbial abundance and activity (microbial biomass, PLFAs, extracellular enzyme activity) and use of UKCEH isotope analysis (Picarro CRDS). The student will also benefit from the CASE partnership with FR in Roslin and spend discrete periods of time being trained on soil fauna identification. Overall, the supervisory team are highly experienced in soil ecology and biogeochemistry, with complementary expertise, and with access to a breadth of facilities at the U. of Stirling, UKCEH (Lancaster), and FR (Roslin).

References & further reading

Andriuzzi, W. S., & Wall, D. H. (2018). Soil biological responses to, and feedbacks on, trophic rewilding. Philosophical Transactions of the Royal Society B: Biological Sciences, 373(1761), 20170448.
Bardgett, R. D., & Van Der Putten, W. H. (2014). Belowground biodiversity and ecosystem functioning. Nature, 515(7528), 505-511.
Bossio, D. A., Cook-Patton, S. C., Ellis, P. W., Fargione, J., Sanderman, J., Smith, P., … & Griscom, B. W. (2020). The role of soil carbon in natural climate solutions. Nature Sustainability, 3(5), 391-398.
Friggens, N. L., Hester, A. J., Mitchell, R. J., Parker, T. C., Subke, J. A., & Wookey, P. A. (2020). Tree planting in organic soils does not result in net carbon sequestration on decadal timescales. Global Change Biology, 26(9), 5178-5188.
Minasny, B., Malone, B. P., McBratney, A. B., Angers, D. A., Arrouays, D., Chambers, A., … & Winowiecki, L. (2017). Soil carbon 4 per mille. Geoderma, 292, 59-86.
Warner, E., Hector, A., Brown, N., Green, R., Savory, A., Gilbert, D., … & Lewis, O. T. (2021). The response of plants, carabid beetles and birds to 30 years of native reforestation in the Scottish Highlands. Journal of Applied Ecology, 58(10), 2185-2194.

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